• Aims：Using droplet microfluidics as a carrier, a highly efficient and stable Raman flow cytometry platform is established by optimizing the coupling between the droplet microfluidic system and the line-shaped laser beam, achieving a single-cell live detection accuracy of ≥ 90% and a sorting throughput of ≥ 200 cells/h.
• Methods: This study explores the adaptability and sensitivity differences of different optical detection methods (e.g., Raman spectroscopy, fluorescence imaging, light scattering) in microscale environments, thereby constructing a high-throughput, high-sensitivity microfluidic optical detection platform.

Develop a microfluidic single-cell sorting platform that integrates gravity-driven mechanisms with optical tweezers to enable high-throughput, non-destructive, and precise manipulation and sorting of single cells.

To address the issues of low accuracy and large sample consumption in traditional biopharmaceutical viscosity measurement, this study proposes a novel microfluidic detection system with high precision, low sample use, and low cost.
• Innovative design of a variable cross-section chip using photocuring 3D printing.
• Multi-channel high-throughput system enabled by parallel gas channel design.

In this study, a variable cross-section microfluidic chip structure was designed and successfully manufactured by photocuring 3D printing technology. A digital-printed (DP) microfluidic viscometer was realized by a pressure-driven flow combined with optical imaging.

Viscosity, as a crucial property of liquids, plays a vital role in various fields, including food, chemical, pharmaceutical, personal care, and biomedicine. Therefore, it is of great significance to develop methods that can accurately measure the viscosity of liquids in various environments. To this end, researchers have developed a variety of viscosity measurement techniques. In view of the complexity of viscosity measurement, viscosity measurement in many cases depends on optical technology. Optical-based viscosity measurement technology has demonstrated excellent performance at both macro and micro levels because of its suitability for low sample volumes and the advantages of non-contact measurement. Besides, it can be easily combined with other technologies and is suitable for a wide range of application scenarios.